The illustra™ triplePrep Kit is designed for the rapid, simultaneous extraction and isolation of high yield genomic DNA (gDNA), total RNA, and total denatured proteins from undivided animal tissues and mammalian cells. The streamlined workflow reduces the overall number of steps, enabling the preparation of all three analytes in less than one hour.
- High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing
- Demonstrated application to various types of whole blood including human, horse, rabbit, rat, and mouse
- Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
- Verified in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
The purification process involves minimal shearing resulting in the production of 5 to 10 μg of good quality, intact genomic DNA from a 200 μl sample. Input sample volumes can vary from 50 μl to 1 ml. The method uses a simple genomic DNA purification protocol that uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a column-based, novel silica membrane. The elution volume can be modified as necessary to obtain the appropriate genomic DNA concentration for most downstream molecular biological applications.
Información para pedidos: Kit contains lysis buffer type 15 (75 ml), wash buffer type 6 (12 ml), elution buffer type 5 (12 ml), elution buffer type 9 (7.5 ml), protein precipitation buffer type 1 (2×17.5 m)l, one vial DNase I, DNase reaction buffer type 1 (1.4 ml), illustra Mini columns (50 x DNA), illustra Mini columns (50 x RNA), collection tubes (200) and an instruction booklet.
Información de suministro: Kit contains illustra blood genomicPrep spin columns prepacked with a novel silica membrane, lyophilised proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
Precaución: Yields and purities of gDNA, total RNA, and total denatured proteins can vary with user and depend on the nature and condition of the input sample.
