IBI's Viral Nucleic Acid Extraction Kit was designed specifically for purification of viral DNA/RNA from cell-free samples; such as serum, plasma, body fluids, and the supernatant of viral infected cell cultures
- Sample Size: 200 µl sample (plasma, serum, body fluid, or supernatant of viral infected cell cultures)
- Operation Time: 40 min or less
- Elution Volume: 50 µl
The Viral Nucleic Acid Extraction Kit was designed specifically for purification of viral DNA/RNA from cell-free samples such as: serum, plasma, body fluids, and the supernatant of viral infected cell cultures. DNA/RNA viruses are lysed quickly and efficiently using the lysis buffer, which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates an optimum condition for nucleic acid binding to the glass fiber of the column. Contaminants such as salts, metabolites, and soluble macromolecular cellular components are removed in the wash process. The nucleic acids are eluted in RNase-Free water and are then ready for use in subsequent reactions including Real-Time PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions. The detection limit for certain viruses depends on the sensitivity of the individual PCR or RT-PCR assays.
Certificaciones: For research use only.
Precaución: Components contain irritant agents. Always wear a lab coat, disposable gloves, and protective goggles during use.
